Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / rat / human Substance P (rabbit polyclonal), Cy3 , Bioss USA , Cat # bs-0064R-Cy3.

Techniques: Functional Assay, Recombinant, Staining, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, SYBR Green Assay, Activation Assay, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software

Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / rat / human NK1R extracellular (rabbit polyclonal), ATTO488 , Alomone Labs , Cat # ATR-001-AG.

Techniques: Functional Assay, Control, Recombinant, Staining, Saline, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, Reverse Transcription, SYBR Green Assay, Activation Assay, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software

Journal: Oncotarget

Article Title: Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

doi: 10.18632/oncotarget.15421

Figure Lengend Snippet: The IVD tissues of the elderly men were used as the control. Positive immunoreactivity produced a brown coloration. a ., c ., e . Both cytoplasmic and nuclear staining of ERα, ERβ and SP immunoreactivity were observed in the nucleus pulposus cells. b ., d ., f . No staining could be observed when the antibody was pre-incubated with a blocking peptide. Bar represents 50 μm; SP, Substance P.

Article Snippet: After the nonspecific binding was blocked with normal 5% goat serum (Vector, S-1000), slides were incubated overnight with a rabbit anti-Substance P antibody (Boster, BA0126, 1:100 dilution), followed by the Alexa Fluor ® 594 Goat anti-rabbit (Invitrogen, A11037) incubation 30 min at room temperature in dark, washed with PBS 3*3 times.

Techniques: Produced, Staining, Incubation, Blocking Assay

Journal: Oncotarget

Article Title: Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

doi: 10.18632/oncotarget.15421

Figure Lengend Snippet: a ., b . Both cytoplasmic and nuclear staining of ERα and ERβ immunoreactivity were observed in the nucleus pulposus cells. c . SP immunoreactivity presented with a cytoplasmic and nuclear staining. Bar represents 50 μm; SP, Substance P.

Article Snippet: After the nonspecific binding was blocked with normal 5% goat serum (Vector, S-1000), slides were incubated overnight with a rabbit anti-Substance P antibody (Boster, BA0126, 1:100 dilution), followed by the Alexa Fluor ® 594 Goat anti-rabbit (Invitrogen, A11037) incubation 30 min at room temperature in dark, washed with PBS 3*3 times.

Techniques: Staining

Journal: Oncotarget

Article Title: Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

doi: 10.18632/oncotarget.15421

Figure Lengend Snippet: Photomicrographs showed SP colocalized with ERα and ERβ at the cytoplasm and nuclear in the nucleus pulposus cells. Bar represents 50 μm; SP, Substance P.

Article Snippet: After the nonspecific binding was blocked with normal 5% goat serum (Vector, S-1000), slides were incubated overnight with a rabbit anti-Substance P antibody (Boster, BA0126, 1:100 dilution), followed by the Alexa Fluor ® 594 Goat anti-rabbit (Invitrogen, A11037) incubation 30 min at room temperature in dark, washed with PBS 3*3 times.

Techniques:

Journal: Oncotarget

Article Title: Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

doi: 10.18632/oncotarget.15421

Figure Lengend Snippet: a . ERα staining negatively correlated with SP expression in IVD tissue. b . SP staining was negatively associated with ERβ. SP, Substance P.

Article Snippet: After the nonspecific binding was blocked with normal 5% goat serum (Vector, S-1000), slides were incubated overnight with a rabbit anti-Substance P antibody (Boster, BA0126, 1:100 dilution), followed by the Alexa Fluor ® 594 Goat anti-rabbit (Invitrogen, A11037) incubation 30 min at room temperature in dark, washed with PBS 3*3 times.

Techniques: Staining, Expressing